=========================== Revio & Vega file structure =========================== The Revio and Vega platforms have a different output file structure than Sequel II and Sequel IIe. Directory structure =================== .. code-block:: Transfer_Scheme_Path/ └─ rNNNNN_YYYYMMDD_HHMMSS/ └─ P_WNN/ ├─ fail_reads/ ├─ hifi_reads/ ├─ metadata/ ├─ pb_formats/ └─ statistics/ Movie Context ID is defined as .. code-block:: mNNNNN_YYMMDD_HHMMSS_sN in particular:: mNNNNN - instrument identifier YYMMDD - date stamp HHMMSS - time stamp sN - stage number (Revio has four) File structure ============== Barcoded data example (from Revio) ---------------------------------- In the following example, one barcode ('bc2001') has been used. If you see the 'default' file infix, this is the standard, unbarcoded TC6 SMRTbell adapter. .. code-block:: Transfer_Scheme_Path/ └─r84001_20220722_134701 └─1_A01 ├─ fail_reads │ ├─ m84001_220722_134701_s1.fail_reads.bc2001.bam │ ├─ m84001_220722_134701_s1.fail_reads.bc2001.bam.pbi │ ├─ m84001_220722_134701_s1.fail_reads.unassigned.bam │ └─ m84001_220722_134701_s1.fail_reads.unassigned.bam.pbi ├─ hifi_reads │ ├─ m84001_220722_134701_s1.hifi_reads.bc2001.bam │ ├─ m84001_220722_134701_s1.hifi_reads.bc2001.bam.pbi │ ├─ m84001_220722_134701_s1.hifi_reads.unassigned.bam │ └─ m84001_220722_134701_s1.hifi_reads.unassigned.bam.pbi ├─ metadata │ ├─ m84001_220722_134701_s1.barcodes.fasta │ ├─ m84001_220722_134701_s1.basecaller.log │ ├─ m84001_220722_134701_s1.ccs.log │ ├─ m84001_220722_134701_s1.darkcal.log │ ├─ m84001_220722_134701_s1.fail_reads.alarms.json │ ├─ m84001_220722_134701_s1.fail_reads.lima.log │ ├─ m84001_220722_134701_s1.fail_reads.primrose.log │ ├─ m84001_220722_134701_s1.hifi_reads.alarms.json │ ├─ m84001_220722_134701_s1.hifi_reads.lima.log │ ├─ m84001_220722_134701_s1.hifi_reads.primrose.log │ ├─ m84001_220722_134701_s1.metadata.xml │ ├─ m84001_220722_134701_s1.ppa.log │ ├─ m84001_220722_134701_s1.run.metadata.xml │ ├─ m84001_220722_134701_s1.sts.xml │ └─ m84001_220722_134701_s1.transferdone ├─ pb_formats │ ├─ m84001_220722_134701_s1.consensusreadset.xml │ ├─ m84001_220722_134701_s1.fail_reads.bc2001.consensusreadset.xml │ ├─ m84001_220722_134701_s1.fail_reads.consensusreadset.xml │ ├─ m84001_220722_134701_s1.fail_reads.unassigned.consensusreadset.xml │ ├─ m84001_220722_134701_s1.hifi_reads.bc2001.consensusreadset.xml │ ├─ m84001_220722_134701_s1.hifi_reads.consensusreadset.xml │ └─ m84001_220722_134701_s1.hifi_reads.unassigned.consensusreadset.xml └─ statistics ├─ m84001_220722_134701_s1.ccs_reports.json ├─ m84001_220722_134701_s1.fail_reads.5mc_report.json ├─ m84001_220722_134701_s1.fail_reads.lima_counts.txt ├─ m84001_220722_134701_s1.fail_reads.lima_guess.json ├─ m84001_220722_134701_s1.fail_reads.lima_report.txt ├─ m84001_220722_134701_s1.fail_reads.lima_summary.txt ├─ m84001_220722_134701_s1.hifi_reads.5mc_report.json ├─ m84001_220722_134701_s1.hifi_reads.lima_counts.txt ├─ m84001_220722_134701_s1.hifi_reads.lima_guess.json ├─ m84001_220722_134701_s1.hifi_reads.lima_report.txt ├─ m84001_220722_134701_s1.hifi_reads.lima_summary.txt └─ m84001_220722_134701_s1.zmw_metrics.json.gz Non-barcoded data example (from Vega) ------------------------------------- .. code-block:: Transfer_Scheme_Path/ └─r21012_20241030_183049 └─1_A01 ├─ fail_reads | ├─ m21012_241030_210509.fail_reads.bam │ └─ m21012_241030_210509.fail_reads.bam.pbi ├─ hifi_reads │ ├─ m21012_241030_210509.hifi_reads.bam │ └─ m21012_241030_210509.hifi_reads.bam.pbi ├─ metadata │ ├─ m21012_241030_210509.basecaller.log │ ├─ m21012_241030_210509.baz2bam.log │ ├─ m21012_241030_210509.ccs.log │ ├─ m21012_241030_210509.combined_baz2bam.log │ ├─ m21012_241030_210509.fail_reads.jasmine.log │ ├─ m21012_241030_210509.fail_reads.samtools.log │ ├─ m21012_241030_210509.fail_reads.trim.log │ ├─ m21012_241030_210509.finalize-dataset.log │ ├─ m21012_241030_210509.hifi_reads.jasmine.log │ ├─ m21012_241030_210509.hifi_reads.samtools.log │ ├─ m21012_241030_210509.hifi_reads.trim.log │ ├─ m21012_241030_210509.metadata.xml │ ├─ m21012_241030_210509.sts.xml │ ├─ m21012_241030_210509.transferdone │ └─ m21012_241030_210509.transferfail ├─ pb_formats │ └─ m21012_241030_210509.hifi_reads.consensusreadset.xml └─ statistics ├─ m21012_241030_210509.ccs_report.json ├─ m21012_241030_210509.ccs_report.txt ├─ m21012_241030_210509.fail_reads.5mc_report.json ├─ m21012_241030_210509.hifi_reads.5mc_report.json ├─ m21012_241030_210509.pbreports.log ├─ m21012_241030_210509.report.pdf ├─ m21012_241030_210509.reports.zip ├─ m21012_241030_210509.summary.json ├─ m21012_241030_210509.zmw_metrics.csv.gz └─ m21012_241030_210509.zmw_metrics.json.gz